蛋白组学是通过生化的方法研究细胞内全部蛋白质组成和动态变化的一门新兴学科 ,在医学生物学研究领域的各个方面都具有巨大的潜力 ,尤其为肿瘤研究提供了崭新的思路与技术。 2DE MS蛋白组学研究技术 ,包括蛋白质标本准备、蛋白质分离纯化、蛋白分析鉴定及数据库等方面 ,目前已应用于肿瘤的发病机制探讨、早期诊断及疗效评价 Proteomics is a new discipline that studies the whole protein composition and dynamic changes in cells through biochemical methods. It has great potential in all aspects of medical biology research, especially providing new ideas and technologies for tumor research. 2DE MS proteomics research techniques, including preparation of protein samples, protein separation and purification, protein analysis and database identification, has been applied to explore the pathogenesis of cancer, early diagnosis and evaluation of the efficacy