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目的：：研究改变制备工艺后三黄烧伤灵对几种常见细菌的体外抑菌效果。方法：采用乙醇回流提取、水提取的混合提取新工艺制备三黄烧伤灵。采用微量肉汤稀释法测定改变制备工艺后三黄烧伤灵对大肠埃希菌、铜绿假单胞菌、金黄色葡萄球菌及乙型副伤寒沙门菌的最小抑菌浓度,对新、旧工艺制备三黄烧伤灵的抑菌效果进行比较,并采用纸片法检测抑菌圈。结果：新工艺制备的三黄烧伤灵对大肠埃希菌、铜绿假单胞菌、金黄色葡萄球菌及乙型副伤寒沙门菌抗菌效果优于旧制备工艺,新工艺的最小抑菌浓度分别为16,16,8,16μg·ml-1,旧工艺的最小抑菌浓度分别为32,64,16,64μg·ml-1。结论：改变制备工艺后制得的三黄烧伤灵抗菌效果明显,有一定的临床使用意义。“,”Objective: To study the antibacterial activity in vitro of Sanhuang Shaoshangling on several common bacteria after changing the preparation process. Methods:Using the new technology of ethanol reflux extraction and water extraction, Sanhuang Sha-oshangling was prepared. A micro-broth dilution method was used to determine the minimum inhibitory concentration ( MIC) of San-huang Shaoshangling for escherichia coli, pseudomonas aeruginosa, staphylococcus aureus and type B paratyphoid salmonella after chan-ging the preparation process. The antibacterial effect of Sanhuang Shaoshangling prepared by the new and the old technology was com-pared, and the inhibition zone was detected by a paper strip method. Results:The antibacterial effect of Sanhuang Shaoshangling pre-pared by the new technology on escherichia coli, pseudomonas aeruginosa, staphylococcus aureus and type B paratyphoid salmonella was better than that of the products prepared by the old technology with the minimum inhibitory concentration ( MIC) of 16 μg·ml-1 , 16μg·ml-1 , 8 μg·ml-1 and 16 μg·ml-1 , respectively, while that of the product prepared by the old technology was 32 μg·ml-1 , 64 μg·ml-1 , 16 μg·ml-1 and 64 μg·ml-1 , respectively. Conclusion: The antibacterial effect of Sanhuang Shaoshangling after changing the preparation process is more significant, which shows certain clinical significance.