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目的:对云南美登木内生真菌Botryosphaeria sp.MHF的化学成分进行研究。方法:采用反相、正相等多种柱色谱法进行分离;应用波谱技术进行结构鉴定。结果:从云南美登木内生真菌B.sp.MHF的发酵物中分离得到8个化合物:分别是麦角甾-5-烯-3-醇(1)、麦角甾-4,6,8,22-四烯-3-酮(2)、麦角甾-3β,5α,9α-三羟基-7,22-二烯-6-酮(3)、麦角甾-7,22-二烯-3β,5α,6β-三醇(4)、麦角甾-5α,8α-环二氧-6,22-二烯-3-醇(5)、fusaproliferin(6)、脑苷脂C(7)和3,4,5-三羟基-四氢萘酮(8)。结论:所有化合物均为首次从以Murashige-Skoog培养基培养的该菌株中分离得到。
Objective: To study the chemical constituents of endophytic fungi Botryosphaeria sp. Methods: Various phases were separated by reversed-phase, positive-phase and column chromatographic methods. Results: Eight compounds were isolated from the fermentation of B.sp.MHF from the endophytic fungi of Mentougum yunnanensis: ergostone-5-en-3-ol (1), ergostat- (2), ergostostine-3β, 5α, 9α-trihydroxy-7,22-dien-6-one (3), ergosta-7,22-diene-3β, (5), fusaproliferin (6), cerebroside C (7), and 3,4 , 5-trihydroxy-tetralone (8). Conclusion: All compounds were isolated from this strain cultured in Murashige-Skoog medium for the first time.