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目的验证生山栀-冬虫夏草复方的抗急性呼吸窘迫综合征(acute respiratory distress syndrome,ARDS)作用。方法 48只新西兰纯种大白兔随机分成空白对照组、油酸对照组与复方低剂量治疗组(0.125g·kg~(-1).d~(-1))及复方中剂量治疗组(0.25g·kg~(-1).d~(-1))、复方高剂量治疗组(1.25g·kg~(-1).d~(-1))5组,各治疗组于造模前3天开始预给药,空白对照组、油酸对照组则予等量的生理盐水,用静脉注射油酸的方法复制 ARDS动物模型,观察各组动脉血气动态变化、各组之间肺系数、肺湿重/干重比、支气管肺泡灌洗液(bronchoalveolarlavage fluid,BALF)白蛋白含量的差异。结果油酸对照组动脉氧分压和氧饱和度进行性下降,肺系数、肺湿重/干重比、BALF 白蛋白含量显著高于空白对照组,而复方高剂量治疗组的动脉血氧饱和度各时间点均高于油酸对照组,肺系数、肺湿重/干重比、BALF 白蛋白含量明显低于油酸对照组,复方低剂量和复方中剂量疗效不明显。结论静脉注射油酸复制 ARDS 模型的方法可靠,预防性使用较大剂量的生山栀-冬虫夏草复方能减轻油酸对家兔的肺损伤,减轻渗出性肺水肿的程度,提高血氧饱和度,对 ARDS 有明显的防治作用。
Objective To evaluate the effect of Shenghayin and Cordyceps sinensis compound on acute respiratory distress syndrome (ARDS). Methods Forty-eight New Zealand purebred white rabbits were randomly divided into control group, oleic acid control group and compound low-dose treatment group (0.125g·kg -1 .d -1) and compound medium-dose treatment group (0.25 g·kg~(-1).d~(-1)), compound high-dose treatment group (1.25g·kg~(-1).d~(-1)) 5 groups, each treatment group before modeling Predose was started on the 3rd day. The control group and the oleic acid control group received the same amount of physiological saline. The animal model of ARDS was duplicated by intravenous injection of oleic acid. The arterial blood gas dynamic changes in each group and the lung coefficient between the groups were observed. Wet weight/dry weight ratio of lung, albumin content in bronchoalveolar lavage fluid (BALF). Results The arterial oxygen partial pressure and oxygen saturation were progressively decreased in the oleic acid control group. The lung coefficient, lung wet/dry weight ratio, and BALF albumin content were significantly higher than those in the blank control group, whereas the arterial oxygen saturation in the compound high dose treatment group was significant. At each time point, it was higher than the oleic acid control group. The lung coefficient, lung wet/dry weight ratio and BALF albumin content were significantly lower than the oleic acid control group. The efficacy of the compound low dose and compound medium dose was not obvious. Conclusion The method of intravenous injection of oleic acid to replicate the ARDS model is reliable. The prophylactic use of higher doses of Hawthorn and Cordyceps sinensis compound can reduce the lung injury of oleic acid to rabbits, reduce the degree of exudative pulmonary edema, and increase blood oxygen saturation. , It has obvious prevention and treatment of ARDS.