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凤眼莲(Eickhornia crassipes)是雨久花科的水生植物,又名凤眼蓝、水葫芦、水浮莲。它在植物学实验教学中用途非常广泛。一、观察细胞有丝分裂凤眼莲的不定根数量极多,当根长约2厘米时,从顶端剪取0.5—1厘米长的一段,去掉根冠,在卡诺固定液中固定1—24小时,以70%酒精洗涤后放入60℃的1N盐酸液内离解12—15分钟,或在室温下用95％酒精和盐酸的配制液(配比为1:1)离解10—12分钟,取出材料水洗干净后,用醋酸或改良的石碳酸品红染色剂染色数分钟并压片,在显微镜下可见到细胞有丝分裂的各个时期。
Eichhornia crassipes (Eickhornia crassipes) is a long hydrangea aquatic plants, also known as Fengye Blue, water hyacinth, water lettuce. It is widely used in botany experiment teaching. First, observe the cell mitosis Edeletar adventitious root number is very large, when the root length of about 2 cm, cut from the top 0.5-1 cm long section, remove the root crown, fixative in the Cano fixed fluid for 1-24 hours, After washing with 70% alcohol, it is allowed to dissociate in 1N hydrochloric acid solution at 60 ° C for 12 to 15 minutes or at room temperature for 10 to 12 minutes with 95% alcohol and hydrochloric acid solution (1: 1 ratio) After washing, the cells were stained with acetic acid or modified fuchsine for several minutes and compressed, and the various stages of cell mitosis were observed under a microscope.