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在大鼠实验性脑梗塞模型上，应用ＭＲＩ灰阶定量以及近红外光谱方法探讨脑梗塞早期诊断的可能性。方法：用尼龙丝插入大鼠一侧颈内动脉造成局灶性脑梗塞，再用Ｔ２加权的ＭＲＩ影像进行灰阶定量测定以及ＨａｍａｍａｔｓｕＮＩＲＯ－５００型近红外光谱仪观测脑血氧代谢的动态变化过程。结果：在缺血３０分钟后，用近红外光谱仪已可测出脑缺血侧的氧合血红蛋白和总血红蛋白量显著降低，还原型血红蛋白增加；在Ｔ２加权的ＭＲＩ影像灰阶定量研究中，缺血２０分钟后缺血区已可见灰阶轻度增强，缺血２小时后，缺血区的Ｔ２加权灰阶值较对照区增加５．５６％，缺血２０小时后，灰阶值较对照侧增加３７．８％。动物实验结果表明，用近红外光谱检测方法结合ＭＲＩ的Ｔ２加权灰阶定量，有可能对脑梗塞作出较早期的诊断。
In the rat experimental cerebral infarction model, MRI gray scale quantitative and near infrared spectroscopy methods to explore the possibility of early diagnosis of cerebral infarction. METHODS: Focal cerebral infarction was induced by insertion of nylon filament into the carotid artery of one side of rat. Gray scale was measured with T2-weighted MRI image. The dynamic changes of cerebral blood oxygen metabolism were observed with Hamamatsu NIRO-500 near-infrared spectrometer. Results: After ischemia for 30 minutes, the content of oxyhemoglobin and total hemoglobin in the ischemic side of brain was significantly reduced and the reduced hemoglobin was increased by near infrared spectroscopy. In T2-weighted gray scale quantitative analysis of MRI images, After 20 minutes of blood, the grayscale was obviously increased in the ischemic area. After 2 hours of ischemia, T2 weighted grayscale in the ischemic area increased by 5.56% compared with that in the control area. After 20 hours of ischemia, Side increased by 37.8%. Animal experiments show that using near infrared spectroscopy combined with T2 weighted gray scale quantitative MRI may make an earlier diagnosis of cerebral infarction.