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目的:探讨外源性CO对IIR所致多器官损伤的防治作用.方法:♂Wistar大鼠64只,随机分为8组.A组:假手术对照组,不阻断SMA,其余手术过程同其他组:B组:IIR组,吸入空气.C组分为C1亚组与C2亚组,缺血前10 min吸入浓度分别为100μL/L、250μL/L的CO;D组:分为D1亚组与D2亚组,再灌注开始时吸入浓度为100μL/L、250μL/L的CO;E组:分为E1亚组与E2亚组,再灌注60 min时开始吸入浓度为100μL/L、250μL/L的CO.实验结束时取不同组织电镜下观察超微结构变化、TUNEL法荧光显微镜下观察细胞凋亡情况.结果:与A组相比,B组肠和肺组织超微结构损伤严重、凋亡细胞大量出现.而肝组织损伤较轻、肾组织损伤不明显,他们的凋亡细胞数目变化不明显.与B组相比,C、D、E组肠、肺、肝组织超微结构损伤表现轻,C组比D组、E组表现轻,各组内两亚组表现无明显差别.肠和肺组织中凋亡细胞数目从多到少依次是:B组,E1组,E2组,D1组,D2组,C1组,C2纽,A组(P<0.05).结论:外源性CO对大鼠IIR所致多器官损伤具有防治作用,且浓度为250μL/L的CO比100μL/L的CO作用更明显:缺血前应用比缺血及再灌注后应用作用更明显.外源性CO对IIR所致器官损伤的防治作用可能是通过调控细胞凋亡实现的.
Objective: To investigate the preventive and therapeutic effects of exogenous CO on multiple organ injury caused by IIR.Methods: 64 male Wistar rats were randomly divided into 8 groups.A group: sham operation control group, without blocking SMA, the rest of the surgical procedure was the same Other groups: Group B: Group IIR inhaled air. Group C consisted of C1 subgroup and C2 subgroup. The inhalation concentrations were 100 μL / L and 250 μL / L CO respectively 10 min before ischemia. Group D: Group and D2 subgroup. At the beginning of reperfusion, the inhalation concentration was 100 μL / L and 250 μL / L of CO. Group E: divided into E1 subgroup and E2 subgroup. At 60 min after reperfusion, inhalation concentration was 100 μL / L and 250 μL / L of CO. At the end of the experiment, the ultrastructural changes in different tissues were observed under electron microscope and apoptosis under fluorescence microscope was observed by TUNEL method.Results: Compared with group A, the ultrastructure of intestine and lung tissue in group B was severely damaged, Apoptotic cells appeared in a large amount, but liver tissue lesion was lighter, renal tissue injury was not obvious, and the number of apoptotic cells did not change obviously.Compared with group B, the ultrastructure of intestine, lung and liver in group C, D and E The damage in group C was lighter than that in group D and group E, and there was no significant difference between the two subgroups in each group.The numbers of apoptotic cells in the intestine and lung tissue were in order from most to less: B group, E1 group, E2 , Group D1, group D2, group C1, group C2 and group A (P0.05) .Conclusion: Exogenous CO can prevent and cure multiple organ injury induced by IIR in rats, and the CO concentration of 250 μL / L is higher than 100 μL The effect of CO on the injury of organs induced by IIR may be through the regulation of apoptosis.