乙酰胆硷(ACh)是第一个被确定的中枢神经介质,由于缺乏理想的显示方法,有关中枢神经系统胆硷能通路的真实情况,却远不如单胺类介质通路了解清楚。而中枢胆硷能机制的研究是当前神经科学领域内一个较为活跃的课题,因此,势必要对胆硷能介质的中枢通路作进一步的了解。本文拟就现行的研究中枢胆硷能通路的方法学作一综述和评价。 Acetylcholine (ACh) is the first identified central nervous system. Due to the lack of ideal display methods, the actual situation of the cholinergic pathway in the central nervous system is far less clear than that of monoamine mediators. The central cholinergic mechanism of the current neuroscience field is a more active issue, therefore, it is necessary to cholinergic mediator of the central pathway for further understanding. This article intends to review and evaluate the current methodology for studying central cholinergic pathways.