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Bifonazole(BFZ)系异吡唑诱导剂,化学结构为1-[(4-联苯)-苯-甲基]-1H-异吡唑。据报告,在体内外均有抗真菌作用。作者观察发癣菌在不同浓度BFZ时出现的超微结构变化,现报告如下。材料和方法:用作者单位保存的发癣菌株,在Sabouraud葡萄糖琼脂培养基中培养2周。用微型刮铲将1白金耳菌团分别放入添加0.4~100μg/ml不同浓度BFZ的培养基中,培养48小时作为电镜检查材料;用不添加BFZ的培养基作对照培养。培养后的菌丝用生理盐水洗净,分成1mm~3大小的团块,在磷酸缓冲液(pH7.4)中浸渍后,用2.5%戊二醛预固定1小时,再用1%锇酸(pH7.4%)固定1小时。酒精脱水,环氧树脂混合液包埋、硬化,制成超簿切片后用饱和醋酸铀加温染色,再用Reynold铅染色液行电子染色后,用H-7000型电子显微镜观察并摄影。结果:在添加各种浓度的BFZ培养基中,发癣菌的细胞壁均肥厚和膨胀。在0.4μg/ml时菌丝屈曲而伸长,细胞质无变化。在0.8~1.0μg/ml时,细胞壁与
Bifonazole (BFZ) is an isoflavone inducer with the chemical structure of 1 - [(4-biphenyl) -benzyl-methyl] -1H-isopyrazole. It has been reported that there are anti-fungal effects both in vitro and in vivo. The author observed Trichophyton in different concentrations of BFZ ultrastructural changes occur, are as follows. Materials and Methods: Trichophyton strains preserved in the Author’s Unit were cultured in Sabouraud dextrose agar for 2 weeks. A group of Platycodon grandiflorum were placed in medium supplemented with 0.4 ~ 100μg / ml BFZ with mini-spatula and incubated for 48 hours for electron microscopy. Culture medium without BFZ was used as control. The cultured hyphae were washed with physiological saline and divided into lumps of 1 to 3 in size. After being immersed in a phosphate buffer (pH 7.4), the mycelia were pre-fixed with 2.5% glutaraldehyde for 1 hour and then treated with 1% osmic acid (pH 7.4%) for 1 hour. Alcohol dehydration, epoxy resin mixture embedded, hardened, made of super-book after slicing with saturated uranyl acetate heating staining, and then Reynold lead staining of electronic dyeing, using H-7000 electron microscope observation and photography. Results: Trichophyton cell walls were hypertrophic and dilated in various concentrations of BFZ medium. At 0.4 μg / ml mycelium buckles and elongates without any change in cytoplasm. At 0.8 ~ 1.0μg / ml, cell wall and