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目的 :研究大肠癌转移与p53基因突变及染色体倍性之间的关系。方法 :采用特异合成引物对p53基因 7～ 8外显子进行PCR扩增 ,结合单链构象多态性分析 (SSCP)和银染技术 ,检则 2 2例大肠癌手术标本p53基因的突变 ;用流式细胞仪 (FACS)分析其染色体的倍性。结果 :在有淋巴结转移 10例大肠腺癌中 ,p53基因突变者占 6例 ( 60 % ) ;在无淋巴结转移的 12例大肠腺癌中 ,p53基因突变者占 1例 ( 8 3% )。在有p53基因突变的 7例大肠腺癌中异倍体者 6例 ( 85 7% ) ;无p53基因突变的 15例大肠癌中异倍体者 6例 ( 4 0 % )。在有淋巴结转移的 10例大肠癌中 ,p53基因突变及DNA异倍体共同表达者 5例 ( 50 % ) ;无淋巴结转移的 12例大肠腺癌中p53基因突变及DNA异倍体共同表达者 1例。结论 :大肠癌p53基因突变及DNA异倍体共同表达与淋巴结转移密切相关。
Objective: To study the relationship between the metastasis of colorectal cancer and p53 gene mutation and chromosome ploidy. METHODS: Specific amplification primers were used to amplify the p53 gene exon 7-8, combined with single strand conformation polymorphism analysis (SSCP) and silver staining technique. The mutations of p53 gene in 22 colorectal cancer surgical specimens were detected. The chromosome ploidy was analyzed by flow cytometry (FACS). RESULTS: In 10 cases of colorectal adenocarcinoma with lymph node metastasis, 6 cases (60%) had p53 gene mutations. In 12 cases of colorectal adenocarcinoma without lymph node metastasis, p53 gene mutation accounted for 1 case (83%). Among the 7 cases of colorectal adenocarcinoma with p53 gene mutation, 6 cases (85.7%) were aneuploid, and 15 cases of colorectal cancer without p53 gene mutation were 6 cases (40%). In 10 cases of colorectal cancer with lymph node metastasis, p53 gene mutation and DNA aneuploid co-expression in 5 cases (50%); p53 gene mutation and DNA aneuploid co-expression in 12 cases of colorectal adenocarcinoma without lymph node metastasis 1 case. Conclusion : The p53 gene mutation and DNA aneuploid co-expression in colorectal cancer are closely related to lymph node metastasis.