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为了探讨p,p’-DDT对大肠癌细胞黏附的影响及机制,以肠癌细胞DLD1为试验材料,采用细胞聚集试验、细胞与基质间黏附分析方法,研究了1 nmol/L的p,p’-DDT作用于DLD1细胞48 h后,p,p’-DDT对DLD1细胞-细胞黏附和细胞-基质黏附的影响。通过Real time PCR和Western blot方法检测了细胞黏附关键因子E-cadherin、CD29的表达变化。结果表明:1 nmol/L的p,p’-DDT作用于DLD1细胞48 h后,DLD1细胞-细胞黏附率明显降低(p<0.01),细胞-基质黏附率明显升高(p<0.01);1 nmol/L的p,p’-DDT作用于DLD1细胞48 h后,降低了E-cadherin的表达水平,同时提高了CD29的表达水平。研究表明,p,p’-DDT具有降低DLD1细胞-细胞黏附并提高其细胞-基质黏附的作用。p,p’-DDT可能通过改变黏附关键因子E-cadherin和CD29的表达来影响细胞黏附,进而促进肿瘤细胞的浸润和侵袭。
In order to investigate the effect and mechanism of p, p’-DDT on the adhesion of colorectal cancer cells, the colorectal cancer cell line DLD1 was used to study the effect of 1 nmol / L p, p Effects of p, p’-DDT on DLD1 cell-cell adhesion and cell-matrix adhesion after D-DCT treatment on DLD1 cells for 48 h. Real-time PCR and Western blot were used to detect the expression of E-cadherin and CD29. The results showed that the cell adhesion rate of DLD1 was significantly decreased (p <0.01) and the cell adhesion rate was significantly increased (p <0.01) after treated with 1 nmol / L p, p’-DDT for 48 h. After treated with 1 nmol / L p, p’-DDT for 48 h, the expression of E-cadherin decreased and the expression of CD29 increased. Studies have shown that p, p’-DDT has the effect of reducing DLD1 cell-cell adhesion and enhancing cell-matrix adhesion. p, p’-DDT may affect the adhesion of cells by changing the expression of E-cadherin and CD29, and promote the infiltration and invasion of tumor cells.