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本研究通过基因替换和重组等技术构建重组病毒解释XJ-160病毒单方向血清学反应的分子基础。以实验室构建的XJ-160病毒全感染性克隆为基础获得XJ-160病毒和辛德毕斯病毒(SINV)糖蛋白基因单独和同时相互替换的重组病毒。首先研究重组病毒对细胞及动物感染性和致病性,同时利用微量细胞中和试验方法鉴定引起XJ-160病毒单方向血清学反应的基因区段。研究结果显示XJ-160病毒E2糖蛋白是影响病毒在细胞中的生长速率,空斑形态及对乳鼠致病性的主要因素。中和试验结果显示SIN病毒E2糖蛋白对决定引起XJ-160病毒单方向血清学反应起着重要作用。本研究确定了引起XJ-160病毒单方向血清学反应的基因区段,并为进一步研究XJ-160病毒基因组结构与功能打下了基础。
In this study, we constructed the molecular basis of recombinant virus to explain the unidirectional serological response of XJ-160 virus by gene replacement and recombination techniques. Based on the experimentally constructed full-infectious XJ-160 clones, the recombinant viruses of XJ-160 virus and Sindbis virus (SINV) glycoprotein genes were individually and simultaneously replaced with each other. Firstly, the infectious and pathogenicity of the recombinant virus to the cells and animals was studied. At the same time, the micro-cell neutralization test was used to identify the gene segment that caused unilateral one-way serological reaction of XJ-160 virus. The results show that the XJ-160 virus E2 glycoprotein is the main factor affecting the virus growth rate in the cells, the morphology of the plaque and the pathogenicity of the mice. Neutralization test results show that SIN glycoprotein E2 plays an important role in determining the unidirectional serological response to XJ-160 virus. This study identified the gene segments that cause unilateral one-way serological response to XJ-160 virus and laid the foundation for further study on the structure and function of XJ-160 virus genome.