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描述了由我所在Sprat经典方法基础上加以改进的MRSAPBPs分离方法。包括:(1)细菌的培养;(2)膜蛋白的制备;(3)聚丙烯酰胺凝胶电泳;(4)放射自显影。结果表明改良方法可以获得更为清晰的PBPs分离带
Describes the MRSAPBPs separation method that is improved on the basis of the classical Sprat method that I have. Including: (1) bacterial culture; (2) the preparation of membrane protein; (3) polyacrylamide gel electrophoresis; (4) autoradiography. The results show that the improved method can obtain clearer PBPs