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用酶免疫分析法 (EIA)研究了人血清非特异性 Ig G在抗 - HCV抗体检测中的干扰作用。结果显示 :随着正常健康人血清标本 (阴性血清 )中 Ig G量的增加 ,其 OD值随之增高 ,当标本中的 Ig G含量增加至一定值时即产生假阳性结果。此结果表明非特异性 Ig G与支持材料 (酶标板 )非特异性结合可造成对 EIA实验结果的干扰作用。这就解释了一些学者报导类风湿性关节炎、自身免疫性肝病、高血清免疫球蛋白血症等患者用 EIA法检测抗 - HCV时易出现假阳性的原因
Enzyme immunoassay (EIA) was used to study the interference of human serum nonspecific Ig G in the detection of anti-HCV antibodies. The results showed that with the increase of Ig G in the serum of healthy volunteers (negative serum), the OD values increased. When the Ig G content in the samples increased to a certain value, a false positive result was produced. This result indicates that the nonspecific binding of nonspecific Ig G to support material (microtiter plate) can cause interference with EIA experimental results. This explains why some scholars report false positives when testing for anti-HCV using EIA in patients with rheumatoid arthritis, autoimmune liver disease, and high serum immunoglobulinuria