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根据南方菜豆花叶病毒（ＳＢＭＶ）两株系Ｂ和Ｃ的核酸序列设计两对引物，利用ＲＴ－ＰＣＲ可以特异地区分纯化的和０．２ｇ病叶中的两株系，ＲＴ－ＰＣＲ检测ＳＢＭＶ－Ｂ的灵敏度为１０ｐｇ。
Two pairs of primers were designed according to the nucleotide sequences of B and C of two strains of southern bean bean mosaic virus (SBMV). The two strains in the purified and 0.2 g diseased leaves could be distinguished by RT-PCR. The SBMV -B has a sensitivity of 10 pg.