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目的 为探讨庚型肝炎病毒(HGV)抗原在肝组织中的定位,进一步研究HGV引起肝脏损害的机理。方法 采用HGVNS_5区抗原制备单克隆抗体(McAb),对4例经临床和病理确诊的血清HGVRNA阳性的慢性庚型肝炎患者肝活检标本,进行免疫组化观察。结果 有1例呈阳性染色,光镜下观察见特异染色大部分定位于肝细胞胞浆中,部分有核着色和膜型染色,呈黄色或棕黄色颗粒状,阳性细胞数量少,大多散在分布,部分呈灶性分布。结论 HGV抗原可在肝细胞中定位,呈胞浆型,部分呈核型和膜型,肝脏病理损害轻,发展慢。
Objective To investigate the localization of Hepatitis G virus (HGV) antigen in liver tissue and to further investigate the mechanism of HGV-induced liver damage. Methods Monoclonal antibody (McAb) was prepared by using HGVNS 5 antigen and immunohistochemistry was performed in 4 liver biopsy specimens from patients with chronic HGV RNA positive chronic hepatitis G confirmed by clinical and pathological examination. The results of a positive staining, observed by light microscopy, most of the specific staining located in the cytoplasm of liver cells, some nuclear staining and membrane-type staining, yellow or brown granular, positive cells in small numbers, mostly scattered distribution , Some were focal distribution. Conclusion HGV antigen can be located in hepatocytes, cytoplasm, some karyotype and membrane type, liver pathological damage light, slow development.