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用多元回归分析73例骨髓移植的重型再生障碍性贫血(SAA)患者,显示移植排斥与植入的骨髓细胞量少于3×10~8个有核细胞/kg 有明显关系。但测定骨髓有核细胞仅能粗略估计输入干细胞的数量,本文测定粒系集落形成细胞(CFU-c),试图探索输入的CFU-c 量与移植成败的关系。病人和方法 10例均符合 SAA 标准,其中原发性7例,肝炎后1例,2例属骨髓再生障碍期的阵发性睡眠性血红蛋白尿。均以环磷酰胺(60mg/kg 5和4天)和800rad(1天)全身照射作移植前准备.采用1970年 Pike 和 Robinson 的方法测定 CFU-c。供者骨髓 CFU-c 从输入的骨髓中取样测定。培养(?)天.结果所有患者在9～14天骨髓重建,无1例移植排斥。平均15天(11～18天)可见到中性粒细胞在血中重现,22天(14～30天)达1×10~9/L,为供者平均献出其骨髓有核细胞总数的2.4±0.4%或 CFU-c总数的1.50±0.2%。移植前受者 CFU-c 极低,移植时接受3.5±0.4×10~8个有核细胞/kg 或1.19±0.32×10~5CFU-c/kg。移植后第10天骨髓 CFU-c 变
Multiple regression analysis of 73 patients with severe aplastic anemia (SAA) who underwent bone marrow transplantation showed a significant relationship between graft rejection and the number of implanted bone marrow cells less than 3 × 10 ~ 8 nucleated cells / kg. However, the measurement of bone marrow nucleated cells can only roughly estimate the number of input stem cells. In this paper, we measured the number of CFU-c in CFU-c and explored the relationship between the amount of CFU-c and the success of transplantation. The patients and methods all met SAA criteria in 10 cases, including 7 cases of primary, 1 case of hepatitis and 2 cases of paroxysmal nocturnal hemoglobinuria with bone marrow aplastic. Pretreatment preparation was performed with cyclophosphamide (60 mg / kg at 5 and 4 days) and 800 rad (1 day) whole body irradiation. CFU-c was determined using the method of Pike and Robinson in 1970. Donor bone marrow CFU-c was sampled from the input bone marrow. (?) Days.Results All patients had bone marrow reconstructions at 9 to 14 days, and none of them had transplant rejection. On the average of 15 days (11-18 days), neutrophils could be seen in the blood, and reached the level of 1 × 10 ~ 9 / L in 22 days (14-30 days), giving the average number of bone marrow nucleated cells Of 2.4 ± 0.4% or 1.50 ± 0.2% of the total CFU-c. Before transplantation, CFU-c was extremely low and received 3.5 ± 0.4 × 10 ~ 8 nucleated cells / kg or 1.19 ± 0.32 × 10 ~ 5CFU-c / kg. Bone marrow CFU-c changes on the 10th day after transplantation