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目的:研究正常大鼠与血瘀模型大鼠灌胃给予淫藤骨痹康方后,血浆中丹参酮ⅡA的浓度变化及丙二醛浓度变化的药动学-药效学(PK-PD)相关性。方法:以氯雷他定为内标,采用HPLC-UV法测定不同时间血浆中丹参酮ⅡA的浓度;采用TBA法测定不同时间血浆中MDA水平;采用梯形面积法计算血药浓度-时间曲线下面积(AUC)与抗氧化效应-时间曲线下面积(AUE);以AUE为Y,lgAUC为X,进行相关与回归分析。结果:在2种不同生理模型下,淫藤骨痹康方在所测的各个时间点均能显著降低血浆中MDA水平。AUE-lgAUC的散点图均呈上升趋势;相关回归分析结果为:正常对照组回归方程为Y=53.367X-30.780,相关系数r=0.822,P=0.007;血瘀模型组回归方程为Y=61.091X-39.863,相关系数r=0.777,P=0.003。结论:淫藤骨痹康方降低体内MDA水平的抗氧化作用与体内丹参酮ⅡA的量呈正相关,丹参酮ⅡA可能为其抗氧化作用的药效物质。
Objective: To study the changes of tanshinone Ⅱ A concentration in plasma and pharmacokinetic-pharmacodynamic (PK-PD) of plasma malondialdehyde (MDA) in normal rats and blood-stasis rats Sex. Methods: The lovastatin was used as the internal standard. The plasma tanshinone Ⅱ A concentration was measured by HPLC-UV method at different time. TBA was used to measure the plasma MDA level at different time. The area under the plasma concentration-time curve was calculated by trapezoidal area method (AUC) and the area under the anti-oxidant effect-time curve (AUE). Correlation and regression analysis were performed with AUE as Y and lgAUC as X. Results: In two different physiological models, JING Teng Gu Bi Kang Fang at various time points measured can significantly reduce the plasma MDA levels. AUE-lgAUC scatter plot showed an upward trend; correlation analysis showed that: the normal control group regression equation Y = 53.367X-30.780, correlation coefficient r = 0.822, P = 0.007; blood stasis model group regression equation Y = 61.091X-39.863, correlation coefficient r = 0.777, P = 0.003. CONCLUSION: Antioxidant effect of JINTIANGUIBIKANG prescription on reducing MDA level in vivo is positively correlated with the amount of tanshinone Ⅱ A in body. Tanshinone ⅡA may be an effective anti-oxidant.