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目的研究晚期糖基化终末产物(AGEs)对体外分离培养的人骨髓间质干细胞(MSCs)增殖的影响。方法采用CCK-8比色法检测在不同时间和浓度AGEs干预下所培养的MSCs的吸光度和增殖活力,同时测定细胞内的丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果 AGEs加入细胞培养后,可显著抑制MSCs的增殖(均P<0.01),并呈剂量和时间依赖效应关系。随着AGEs-BSA作用浓度的增加,细胞内丙二醛含量明显增加,而细胞匀浆中超氧化物歧化酶的活性却受到了抑制(均P<0.01),具有剂量依赖效应。结论 AGEs通过增强MSCs内的氧化应激,破坏MSCs内环境稳定性,从而抑制MSCs的增殖。研究晚期糖基化终末产物(AGEs)对体外分离培养的人骨髓间质干细胞(MSCs)增殖的影响。
Objective To study the effects of advanced glycation end products (AGEs) on the proliferation of human bone marrow mesenchymal stem cells (MSCs) cultured in vitro. Methods The absorbance and proliferation activity of MSCs cultured under different concentrations of AGEs were detected by CCK-8 colorimetric assay. The contents of malondialdehyde (MDA) and superoxide dismutase (SOD) were measured. Results AGEs could significantly inhibit the proliferation of MSCs (all P <0.01) when added into cell culture in a dose-and time-dependent manner. With the increase of the concentration of AGEs-BSA, the content of malondialdehyde (MDA) in the cells increased significantly, while the activity of superoxide dismutase (SOD) in the cell homogenate was inhibited (all P <0.01), with a dose-dependent effect. Conclusion AGEs can inhibit the proliferation of MSCs by enhancing oxidative stress in MSCs and disrupting the stability of MSCs. To investigate the effects of advanced glycation end products (AGEs) on the proliferation of human bone marrow mesenchymal stem cells (MSCs) cultured in vitro.