论文部分内容阅读
目的构建MicroRNA-143慢病毒载体并探讨其在子宫内膜异位发生中的作用及机制。方法分离培养正位和异位子宫内膜基质细胞,检测HOXA10蛋白和MicroRNA-143的表达;构建MicroRNA-143慢病毒载体并感染子宫内膜基质细胞,流式检测HOXA10蛋白的表达。结果正位子宫内膜基质细胞HOXA10蛋白表达的平均荧光强度是异位子宫内膜基质细胞的1.74倍,差异有统计学意义(P<0.05);异位子宫内膜基质细胞内MicroRNA-143显著低于正位子宫内膜基质细胞,下降了64.56%,差异有统计学意义(P<0.05);MicroRNA-143转染可以显著促进异位子宫内膜基质细胞HOXA10蛋白表达,相对于阴性病毒组,平均荧光强度上升了72.03%,差异有统计学意义(P<0.05)。MicroRNA-143转染对正位子宫内膜基质细胞差异无统计学意义(P>0.05)。结论子宫内膜异位症患者MicroRNA-143和HOXA10蛋白表达显著下降,通过增强MicroRNA-143表达可以显著上调HOXA10蛋白表达抑制子宫内膜异位症的发生。
Objective To construct a lentiviral vector containing MicroRNA-143 and investigate its mechanism and mechanism in endometriosis. Methods The normal and ectopic endometrial stromal cells were isolated and cultured to detect the expression of HOXA10 protein and MicroRNA-143. MicroRNA-143 lentiviral vector was constructed and infected with endometrial stromal cells to detect the expression of HOXA10 protein. Results The mean fluorescence intensity of HOXA10 protein expression was 1.74 times that of ectopic endometrial stromal cells (P <0.05), while that of MicroRNA-143 in ectopic endometrial stromal cells was significantly higher than that of ectopic endometrial stromal cells The expression of HOXA10 protein in ectopic endometrial stromal cells was significantly lower than that in normal endometrial stromal cells (P <0.05). MicroRNA-143 transfection decreased the expression of HOXA10 protein in ectopic endometrial stromal cells, , The average fluorescence intensity increased by 72.03%, the difference was statistically significant (P <0.05). MicroRNA-143 transfection of posterior endometrial stromal cells was no significant difference (P> 0.05). Conclusion The expression of MicroRNA-143 and HOXA10 in patients with endometriosis is significantly decreased. The expression of HOXA10 can be significantly up-regulated by enhancing the expression of MicroRNA-143, which can inhibit the occurrence of endometriosis.