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目的 通过舌癌术前化疗细胞增殖和细胞凋亡相关基因Bcl -2表达的研究 ,探讨化疗作用机制。方法 对舌乳头状瘤、舌癌和舌癌术前化疗后标本共 3 4例 ,采用免疫组织化学染色技术 -酶标链亲和素生物素法(LSAB)染色并进行光镜观察。结果 PCNA在舌癌中的表达最强 ,在舌癌术前化疗后的表达明显减弱 ( p <0 .0 5 )与舌乳头状瘤表达相似 ( p >0 .0 5 ) ,Bcl-2在舌癌化疗后及舌乳头状瘤中低表达 ( p >0 .0 5 ) ,舌癌中表达明显增强( p <0 .0 5 )。结论 化疗药物抑制肿瘤生长的机制可能与抑制肿瘤细胞增殖和诱导肿瘤细胞凋亡有关 ,提示舌癌术前化疗对肿瘤的预后有重要意义
Objective To study the mechanism of chemotherapy by studying the cell proliferation and apoptosis-related gene Bcl-2 expression in preoperative chemotherapy of tongue cancer. Methods A total of 34 specimens of tongue papilloma, tongue cancer and tongue cancer were collected before the chemotherapy. The specimens were stained with LSAB and observed with light microscopy by immunohistochemical staining. Results The expression of PCNA was the strongest in tongue cancer and significantly decreased in preoperative chemotherapy of tongue cancer (p <0.05). The expression of PCNA was similar to tongue papilloma (p> 0.05) After tongue cancer chemotherapy and tongue papilloma low expression (p> 0.05), tongue cancer expression was significantly enhanced (p <0.05). Conclusion The mechanism of chemotherapeutic drugs in inhibiting tumor growth may be related to the inhibition of tumor cell proliferation and induction of apoptosis in tumor cells, suggesting that preoperative chemotherapy of tongue cancer is of great significance for the prognosis of the tumor