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以往的研究已经表明分子量为80KD_a的多肽(kp)和恶性疟原虫在感染的红细胞膜上形成小节有关。并且还发现kp和鹇疟原虫的富组氨酸的蛋白质(HRP)具有共同抗原决定因素。现作者用短周期标记法对kp的合成和代谢作进一步研究。作者用~3H-组氨酸分别标记体外培养的有节恶性疟原虫(K~+)和无节恶性疟原虫(K~-),用冻融法破细胞,离出上清与用鹇疟原虫富组氨酸蛋白质免疫所得抗血清制得免疫沉淀物。将所得离心沉淀与免疫沉淀进行SDS—PAGE电泳分析,结果表明2小时标记时,K~+及K~-疟原虫红细胞均出现一分子
Previous studies have shown that polypeptides with a molecular weight of 80 kDa (kp) are associated with the formation of nodules on infected erythrocyte membranes by Plasmodium falciparum. It has also been found that kp and Plasmodium-rich proteins (HRPs) of Plasmodium olava have common antigenic determinants. The author uses the short cycle labeling method kp for further synthesis and metabolism. The authors used ~ 3H-histidine to mark the in vitro culture of Plasmodium falciparum (K ~ +) and Plasmodium falciparum (K ~ -), respectively. Proto-histidine-rich protein immunization obtained antiserum immunoprecipitate. The obtained centrifugal sedimentation and immunoprecipitation SDS-PAGE electrophoresis analysis showed that 2 hours mark, K ~ + and K ~ - Plasmodium red blood cells were a molecule