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本文介绍了试用地高辛标记,将炭疽芽孢杆菌的PXD1和PXD2两质粒上分别扩增到290bp和288bp基因片断制成非放射性标记探针,利用菌落原位杂交方法进行检测,表明两探针的敏感性、特异性均良好,提示该两探针将能为基层工作提供更准确、快速的炭疽杆菌检验技术。
This article describes the trial digoxin markers, the B. anthracis PXD1 and PXD2 two plasmids were amplified to 290bp and 288bp gene fragment made of non-radioactive labeled probe, the use of colony in situ hybridization method for detection, indicating that the two probes The sensitivity and specificity are good, suggesting that the two probes will provide more accurate and rapid Bacillus anthracis test for grassroots work.