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目的:制定五味肝清片质量控制方法。方法:采用薄层色谱法鉴别黄芪和虎杖,采用高效液相色谱法测定样品中大黄素的含量。色谱条件为Diamonsil C18(4.6mm×250mm,5μm)色谱柱;流动相为0.1%磷酸溶液-甲醇(13∶87);流速0.8mL.min-1;检测波长252nm。结果:定性鉴别阴性无干扰,分离度好;大黄素在6.16～61.6mg·L-1范围内与峰面积积分值呈良好的线性关系。结论:该方法简便快速,结果准确、可靠,可用于该制剂的质量控制。
Objective: To develop the method of quality control of Wuwei Hep Gut Tablet. Methods: Astragalus membranaceus and Polygonum cuspidatum were identified by TLC, and the content of emodin in the samples was determined by high performance liquid chromatography. The chromatographic conditions were Diamonsil C18 (4.6mm × 250mm, 5μm) column; the mobile phase was 0.1% phosphoric acid solution-methanol (13:87); the flow rate was 0.8mL.min-1; Results: The qualitative identification of negative non-interference, good separation; emodin in 6.16 ~ 61.6mg · L-1 range and peak area integral value showed a good linear relationship. Conclusion: The method is simple and rapid, accurate and reliable, and can be used for the quality control of the preparation.