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目的:探讨Bmi-1和Mel-18在乳腺癌组织中的表达和临床意义。方法:采用RT-PCR和免疫组织化学染色法检测Bmi-1和Mel-18基因与蛋白在40例乳腺癌组织和20例乳腺良性肿瘤组织及20例正常乳腺组织中的表达。分析两者在结直肠癌组织中的表达情况和相关性,以及两者表达与临床病理因素的关系。结果:Bmi-1 mRNA的表达和蛋白阳性率在正常乳腺组织、乳腺良性组织、乳腺癌组织均呈明显依次递增(均P<0.05),而Mel-18则基本呈反向趋势(正常乳腺组织与乳腺良性组织间差异不明显);乳腺癌组织中Bmi-1与Mel-18 mRNA及蛋白表达之间均明显呈负相关(r=-0.317,P=0.023;r=-0.413,P=0.008);两者基因表达和蛋白阳性率水平与淋巴结转移及临床分期密切相关(均P<0.05),而与患者的年龄、绝经状况、肿瘤大小、组织学分级无关(均P>0.05)。结论:Bmi-1过表达与Mel-18低表达可能是乳腺组织恶性转变以及乳腺癌发生浸润转移的重要生物学标志。
Objective: To investigate the expression and clinical significance of Bmi-1 and Mel-18 in breast cancer. Methods: The expressions of Bmi-1 and Mel-18 gene and protein in 40 breast cancer tissues, 20 benign breast tumor tissues and 20 normal breast tissues were detected by RT-PCR and immunohistochemical staining. To analyze the expression and correlation between the two in colorectal cancer and their relationship with clinicopathological factors. Results: The expression of Bmi-1 mRNA and the positive rate of Bmi-1 mRNA in normal breast tissue, benign breast tissue and breast cancer tissues were significantly increased (all P <0.05), while Mel-18 tended to reverse trend (normal breast tissue And breast benign tissues were not significantly different). There was a significant negative correlation between the expression of Bmi-1 and Mel-18 mRNA and protein in breast cancer (r = -0.317, P = 0.023; r = -0.413, P = 0.008 ). The levels of gene expression and protein positive rate were closely correlated with lymph node metastasis and clinical stage (all P <0.05), but not with age, menopausal status, tumor size and histological grade (all P> 0.05). Conclusion: The overexpression of Bmi-1 and the low expression of Mel-18 may be important biomarkers of malignant transformation of breast tissue and invasion and metastasis of breast cancer.