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层控矿床中形成金属硫化物的还原态硫来自下列作用过程：①在沉积物理藏不深、温度小于90℃的条件下，细菌可将海水流好盐还原；②分散在沉积物中的有机质在热成熟过程中可释放出H2S;③硫酸盐在较高温度（＞100℃）的地下深处通过化学反应可形成非生物H2S。上述三种方式形成的还原态硫具有不同的同位素特征。
The formation of metal sulfide in the stratabound ore deposit results from the following processes: (1) the bacteria can reduce the salinity of the seawater under the condition that the sedimentary physical condition is not deep and the temperature is less than 90 ℃; (2) the organic matter dispersed in sediments H2S can be released during thermal maturation; ③ Sulfate forms non-biological H2S through chemical reaction in the deep underground at higher temperature (> 100 ℃). The reduced sulfur formed by the above three methods has different isotopic characteristics.