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目的:探讨六味地黄丸对APP/PS1双转基因小鼠的学习记忆能力以及胆碱能系统的影响。方法:将40只APP/PS1双转基因小鼠随机分为APP/PS1模型组,六味地黄丸低、中、高剂量(0.59,1.18,2.36 g·kg-1·d-1)组,西药布洛芬(0.04g·kg-1·d-1)组,每组8只。相匹配的3月龄野生型(WT)小鼠8只,作为正常组。ig给药3个月,通过Morris水迷宫检测学习记忆能力。采用酶联免疫吸附测定(ELISA)法检测各组小鼠大脑皮层乙酰胆碱(ACh)含量,乙酰胆碱转移酶(Ch AT)和乙酰胆碱酯酶(ACh E)的活性。结果:与正常组相比,APP/PS1模型组小鼠在各时期找到平台所需的时间(潜伏期)均延长(P<0.05),在平台所在象限停留时间,百分比,穿越平台次数均降低(P<0.05),ACh含量及Ch AT活性均降低(P<0.05)。六味地黄丸低、中、高剂量组和布洛芬组与APP/PS1模型组相比潜伏期缩短(P<0.05),在平台所在象限停留时间,百分比,穿越平台次数均增高(P<0.05),ACh含量及Ch AT活性均增高(P<0.05),ACh E活性无统计学差异。结论:不同剂量的六味地黄丸可通过提高脑内Ch AT活性,增加ACh含量影响中枢神经胆碱能系统,从而改善APP/PS1双转基因小鼠学习记忆能力。
Objective: To investigate the effects of Liuwei Dihuang Pill on learning and memory abilities and cholinergic system in APP / PS1 double transgenic mice. Methods: 40 APP / PS1 double transgenic mice were randomly divided into APP / PS1 model group, low, medium and high doses of Liuweidihuangwan (0.59,1.18,2.36 g · kg-1 · d-1) Lupine (0.04g · kg-1 · d-1) group, 8 rats in each group. Eight matched 3-month-old wild-type (WT) mice were used as the normal group. ig administered 3 months, through the Morris water maze test learning and memory capabilities. The levels of acetylcholine (ACh), acetylcholinesterase (AChE) and acetylcholinesterase (AChE) in cerebral cortex were detected by enzyme linked immunosorbent assay (ELISA). Results: Compared with the normal group, the time required to find the platform in the APP / PS1 model group was prolonged (P <0.05), and the residence time, the percentage and the number of crossing the platform in the quadrant of the platform decreased P <0.05), ACh content and ChAT activity decreased (P <0.05). Compared with APP / PS1 model group, the latent period of Liuweidihuangwan low, middle and high dose group and ibuprofen group was shorter than that of APP / PS1 model group (P <0.05) ACh content and Ch AT activity were increased (P <0.05), ACh E activity was no significant difference. Conclusion: Different dosages of Liuweidihuangwan can improve the learning and memory abilities of APP / PS1 double transgenic mice by increasing ChAT activity in the brain and increasing ACh content in the central nervous system cholinergic system.