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目的 :研究补阳还五汤抗脑缺血后迟发性神经元坏死 (DNN)作用与一氧化氮 (NO)和一氧化氮合酶(NOS)的关系。方法 :采用沙土鼠双侧颈总动脉夹闭脑缺血再灌注模型 ,于再灌注后 48小时取前脑皮质测定NO和 NOS。结果 :缺血再灌注后 48小时脑组织 NO含量和 NOS活性降低。腹腔注射补阳还五汤可增加缺血再灌注 48小时后脑组织 NO含量 ,提高 NOS活性。结论 :脑缺血后 DNN与脑内 NO的生成减少有一定的关系 ,补阳还五汤可能通过调节脑组织 NO的生成而发挥抗脑缺血的作用
Objective: To study the effect of Buyang Huanwu Decoction on the delayed neuronal necrosis (DNN) after cerebral ischemia and the relationship between nitric oxide (NO) and nitric oxide synthase (NOS). METHODS: Cerebral ischemia reperfusion models were established by clamping the bilateral common carotid arteries of gerbils. Forty-eight hours after reperfusion, the forebrain cortex was measured for NO and NOS. Results : NO content and NOS activity in brain tissue decreased 48 hours after ischemia and reperfusion. Intraperitoneal injection of Buyang Huanwu Decoction can increase the content of NO in brain tissue after 48 hours of ischemia-reperfusion and increase NOS activity. Conclusion: DNN has a certain relationship with the reduction of NO production in brain after cerebral ischemia. Buyang Huanwu Decoction may play a role in anti-cerebral ischemia by regulating NO production in brain tissue.