论文部分内容阅读
目的和方法：采用家兔盲肠结扎穿孔（ＣＬＰ）模型观察了ＣＬＰ前后以及一氧化氮（ＮＯ）合成抑制剂左旋硝基精氨酸（Ｌ－ＮＮＡ）对平均动脉血压（ＭＡＰ），肺动脉压（ＰＡＰ），入、出肺血ＮＯ、丙二醛（ＭＤＡ）含量和超氧化物歧化酶（ＳＯＤ）活性的改变。结果：兔ＣＬＰ后１～５ｈＭＡＰ明显下降，而在２、２５ｈ出现ＰＡＰ明显升高。ＣＬＰ前出肺血ＮＯ含量显著低于入肺血，ＣＬＰ后２５ｈ入肺血ＮＯ含量低于ＣＬＰ前，而出肺血ＮＯ含量与ＣＬＰ前相比无明显差异，且入、出肺血ＮＯ含量无明显差异。ＣＬＰ后２５ｈ入肺血ＭＤＡ含量比ＣＬＰ前有明显增加，出肺血无显著改变；而出肺血ＳＯＤ活性比ＣＬＰ前有明显增高，入肺血无显著改变。注入Ｌ－ＮＮＡ后入、出肺血ＮＯ含量明显降低，各时点ＰＡＰ都明显增高，５ｈ生存率降低，同时入、出肺血ＭＤＡ含量明显升高，ＳＯＤ活性明显下降。结论：肺内ＮＯ含量改变可能与氧自由基有关，在兔ＣＬＰ后早期阶段ＮＯ具抗肺动脉高压和抗氧化的作用
Objective and Methods: Rabbit cecal ligation and puncture (CLP) model was used to observe the effects of L-NNA on the mean arterial pressure (MAP) and pulmonary arterial pressure (MAP) before and after CLP, and the effect of nitric oxide synthase inhibitor (L-NNA) PAP), the level of NO in lungs, the content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD). Results: After 1 ~ 5h rabbits CLP significantly decreased PAP, while 2,2 5h PAP significantly increased. The level of NO in pulmonary blood before CLP was significantly lower than that in lungs. The level of NO in lungs after CLP was lower than that in CLP at 25h (P <0.05), while the level of NO in lungs was no significant difference NO content no significant difference. After CLP 25h into the lung blood MDA levels increased significantly than before CLP, no significant changes in pulmonary blood; and out of the lung SOD activity was significantly higher than before CLP, into the lung blood did not change significantly. After injection of L-NNA, the content of NO in lungs and lungs decreased significantly, the PAP in each time point increased obviously, the survival rate decreased in 5h, and the content of MDA in lung and lungs increased significantly and the activity of SOD decreased obviously. CONCLUSION: The change of NO content in the lung may be related to oxygen free radicals. NO may have anti-pulmonary hypertension and anti-oxidative effects in the early stage of rabbit CLP