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本田间试验在ＨｏｋｋａｉｄｏＫｉｔａｍｉ农业试验站进行了三年，旨在确定能否在收获前预测冬小麦籽粒蛋白质含量（ＧＰＣ）。供试材料为适于加工日本面条的冬小麦品种Ｃｈｉｈｏｋｕｋｏｍｕｇｉ。当分级施Ｎ时，根据抽穗末期倒二叶（旗叶下面的那片叶）的颜色可以准确地预测出ＧＰＣ。为了评价这一试验的有效性，在３年试验期间同时调查了Ｈｏｋｋａｉｄｏ东部地区的９５块生产麦田。结果表明，在环境条件不同的许多地点，用倒二叶叶色预测ＧＰＣ的准确性较低，但叶色可以作为一个指数来判断ＧＰＣ相对于加工要求是低还是高。当在抽穗末期用叶绿素仪测得的倒二叶叶色值小于４０时，ＧＰＣ可能低于加工标准。这一指数可以在Ｈｏｋｋａｉｄｏ东部地区采用，但泥炭土田块除外。
The field trial was conducted at the HokkaidoKitami Agricultural Experimental Station for three years to determine whether it is possible to predict winter wheat grain protein content (GPC) prior to harvest. The test material is Chihokukomugi, a winter wheat variety suitable for processing Japanese noodles. When grading N, the GPC can be accurately predicted from the color of the second leaf (the leaf below the flag leaf) at the end of heading. In order to evaluate the effectiveness of this test, 95 production wheat fields in the eastern Hokkaido region were also investigated during the three-year trial. The results show that in many locations with different environmental conditions, the prediction of GPC using inverted leaf color is less accurate, but leaf color can be used as an index to determine whether GPC is low or high relative to processing requirements. When the inverted leaflet color value measured by chlorophyllometry at the end of heading is less than 40, GPC may be lower than the processing standard. This index can be used in the eastern part of Hokkaido, except peat blocks.