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用杂种犬16只分为失血性休克组(HS)和失血性休克人参果皂甙组(HSG)。HS组动物由一侧颈总动脉放血至血压40mmHg(5.33kPa),放血后4h内采用放血和输血的方法维持血压。HSG组动物于放血前30min静脉注射人参果皂甙,HS组动物静脉注射等量的生理盐水,其它实验过程同HS组。两组动物存活时间,处死前血压和终失血量均存在显著性差异(P<0.005,P<0.05,P<0.001)。动物死亡后观察心肌细胞内某些酶的含量变化,HSG组动物心肌磷酸肌酸激酶(CK)、乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)和细胞色素氧化酶(CCO)的含量明显高于HS组。电镜观察:HS组动物心肌细胞的肌膜、核膜和线粒体均有不同程度的损伤,而HSG组动物心肌细胞的肌膜、核膜和线粒体基本正常。以上结果表明:人参果皂甙具有抗休克和保护心肌的作用。
Sixteen hybrid dogs were divided into hemorrhagic shock group (HS) and hemorrhagic shock ginseng fruit saponin group (HSG). Animals in HS group were bled from one common carotid artery to a blood pressure of 40 mmHg (5.33 kPa), and blood pressure was maintained within 4 h after bleeding by bloodletting and transfusion. Animals in HSG group were injected with ginseng saponin intravenously 30 minutes before blood excretion, and animals in HS group were injected intravenously with equal volume of normal saline. The other experimental procedures were the same as those in HS group. There were significant differences in the survival time, blood pressure and final blood loss between the two groups (P<0.005, P<0.05, P<0.001). After the animals died, the contents of certain enzymes in myocardial cells were observed. Myocardial phosphocreatine kinase (CK), lactate dehydrogenase (LDH), succinate dehydrogenase (SDH) and cytochrome oxidase (CCO) were found in HSG animals. The content is significantly higher than the HS group. Electron microscopy showed that the myocardium, nuclear membrane and mitochondria of the myocardial cells in HS rats were damaged in varying degrees, while the myocardium, nuclear membrane and mitochondria of the myocardial cells in HSG animals were normal. The above results show that: ginsenoside has anti-shock and myocardial protection.