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本文用C_(1q)-ELISA法,K-ELISA法和PEG沉淀法对60例不同期、不同型的儿童风湿热患者进行循环免疫复合物(CIC)检测,并以健康儿童和急性肾炎为对照。结果三种方法均显示活动期心脏炎型风湿热的CIC显著增高。两种ELISA法显示急性肾炎的CIC显著增高。K-ELISA法和PEG沉淀法显示活动期心脏炎型风湿热的CIC显著高于非心脏炎型和静止期风湿热。以上检测结果提示CIC的检测可能有助于判断风湿是否活动以及心脏是否受累。另外,本文亦用PEG沉淀结合单向免疫扩散的技术对风湿热CIC的分类作了初步探讨
In this paper, C_ (1q) -ELISA method, K-ELISA method and PEG precipitation method were used to detect circulating immunocomplex (CIC) in 60 children with different stages and different types of rheumatic fever, and healthy children and acute nephritis as control . Results All the three methods showed that the CIC of rheumatic fever with active carditis was significantly higher. Both ELISA methods showed a significant increase in CIC in acute nephritis. K-ELISA and PEG precipitation showed that the CIC of rheumatic fever with active carditis was significantly higher than that of non-cardiac and quiescent rheumatic fever. The above test results suggest that the CIC test may help determine whether rheumatism is active and whether the heart is involved. In addition, this article also PEG precipitation and unidirectional immunodiffusion technology for classification of rheumatic fever CIC made a preliminary study