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目的探讨急性脑梗死急性期患者血小板内游离钙和ＴＸＢ２含量之间的相互关系及意义。方法 用Ｆｕｒａ－２／ＡＭ荧光技术测定了４６例急性脑梗死患者急性期及２５例正常对照组血小板内钙含量，用放射免疫分析法测定其血小板内血栓素Ｂ２（ＴＸＢ２）含量，用比浊法测定其血小板聚集。结果患者血小板内游离Ｃａ２＋和ＴＸＢ２含量均分别明显高于正常对照组（１４７．２６±１０．８５ ｖｓ １１９．３０±１０．９４ ｎｍｏｌ／Ｌ和３．３８±０．７８ｖｓ ４．８８±０．９７ｎｇ／１０９血小板，Ｐ均＜０．０１）；在患者中，低（０．５μｍｏｌ／Ｌ）和高（５μｍｏｌ／Ｌ）浓度二磷酸腺苷（ＡＤＰ）诱导的血小板聚集均明显高于正常对照组（０．４４±０．１９ｖｓ ０．２６±０．１４和０．７３±０．１３ｖｓ０．３９±０．１９，Ｐ均＜０．０１）；在患者中血小板内游离Ｃａ２＋含量和ＴＸＢ２含量均分别与５μｍｏｌ／Ｌ二磷酸腺苷诱导的血小板呈明显的正相关性（ｒ分别为０．７８６和０．５９３，Ｐ均＜０．０１），而血小板内Ｃａ２＋与ＴＸＢ２成明显的正相关性（ｒ为０．６５２，Ｐ＜０．０１）。结论 急性脑梗死患者在急性期血小板功能处于活跃状态，可能与其内 Ｃａ２＋含量增加、ＴＸＢ
Objective To investigate the relationship between platelet intracellular free calcium and TXB2 content in patients with acute cerebral infarction and its significance. Methods The contents of platelet calcium in 46 cases of acute cerebral infarction patients and 25 cases of normal control group were determined by Fura-2 / AM fluorescence assay. The levels of thromboxane B2 (TXB2) in platelets were determined by radioimmunoassay. Method to determine the platelet aggregation. Results The levels of free Ca2 + and TXB2 in platelets of patients were significantly higher than those of the normal control group (147.26 ± 10.85 vs 119.30 ± 10.94 nmol / L and 3.38 ± 0.78 vs 4.88 ± 0, respectively). 97ng / 109 platelet, all P <0.01). In the patients, platelet aggregation induced by low concentration (0.5μmol / L) and high concentration (5μmol / L) of adenosine diphosphate (ADP) was significantly higher than that of the normal control (0.44 ± 0.19vs 0.26 ± 0.14 and 0.73 ± 0.13vs0.39 ± 0.19, P <0.01). In patients with platelet free Ca2 + content and TXB2 content (R = 0.786 and 0.593, respectively, P <0.01), while there was a significant positive correlation between platelet Ca2 + and TXB2 (R = 0.652, P <0.01). Conclusions Patients with acute cerebral infarction in an acute phase of platelet function is active, and may be related to its increased content of Ca2 +, TXB