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用硫酸铵分级沉淀、离子交换 (DEAE Sepharose)和分子筛 (S 2 0 0 )的方法 ,从食用菌杨树菇的子实体中分离提纯了一种具有抑制烟草花叶病毒 (TMV)侵染活性的蛋白质 ,称作为AAVP。用SDS PAGE、IEF等方法分析AAVP ,结果均呈现为单一条带。经SDS PAGE测定其亚基的相对分子质量为 15 .8kD ,IEF PAGE计算其等电点为 3.75。氨基酸组成的分析结果表明 ,AAVP中不含Cys,含有少量的His和Met,而富含酸性氨基酸。AAVP是一种N端焦谷氨酰环化封闭的蛋白质 ,经N端去封闭后测得N端氨基酸序列为QGVNIYNIVAGA。当AAVP的浓度为 2 0 0mg/L时 ,对TMV的抑制率为 84.32 %。而它对供试的 3种植物病原真菌 :绿色木霉、香蕉炭疽菌和甘薯蔓割病菌的菌丝生长没有任何抑制作
Using ammonium sulfate fractionation, ion exchange (DEAE Sepharose) and molecular sieve (S 2 0 0) method, isolated from a fruiting body of the edible fungus poplar mushroom purified with a inhibitory activity against tobacco mosaic virus (TMV) Of the protein, called AAVP. Analysis of AAVP by SDS PAGE, IEF and other methods showed a single band. The relative molecular mass of its subunit was 15.8kD by SDS PAGE and was 3.75 by IEF PAGE. Amino acid composition analysis showed that AAVP does not contain Cys, contains a small amount of His and Met, but rich in acidic amino acids. AAVP is an N-terminal pyroglutaminyl cyclized closed protein. The N-terminal amino acid sequence of the AAVP is QGVNIYNIVAGA after being blocked by the N-terminal. When the concentration of AAVP was 200 mg / L, the inhibition rate of TMV was 84.32%. However, it did not inhibit the mycelial growth of the three phytopathogenic fungi tested: Trichoderma viride, banana anthracnose and sweet potato sp.