一、材料1、百日咳 I 相菌悬液30亿/ml 并加入适量美兰染液。2、所用试管,吸管均干烤灭菌。3、U 型板,0.025ml/滴的标化滴管,二、方法243份血清用两种方法同时测定,其操作程序和结果判定标准均按1983年《中南区计划免疫监测资料汇编》实施。凡效价≥1:320者判为阳性。凡两法结果不符合者均做重复试验后确定之. First, the material 1, pertussis I phase suspension 3 billion / ml and adding the right amount of Meilan dye. 2, the test tube, straw are dry roast sterilization. 3, U-shaped plate, 0.025ml / drop of the standard dropper, two methods 243 serum simultaneously measured by two methods, the operating procedures and the results of the criteria are based on the 1983 “Central South Planned Immunization Monitoring Data Collection” . Where titers ≥ 1: 320 were judged as positive. Where the results of two laws do not meet those who have done after repeated testing.