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目的:研究鸡矢藤提取物(EPS)对链脲佐菌素(STZ)诱导的糖尿病小鼠血糖和抗氧化作用的影响。方法:小鼠尾静脉注射120 mg·kg-1STZ建立糖尿病模型。糖尿病小鼠随机分为5个组,每组10只动物,分别为:模型组,二甲双胍组,鸡矢藤低、中、高剂量组(2.5,5,10 g·kg-1),另设10只正常小鼠作为空白对照组。灌胃给药14 d,并于药前、药后7,14 d测定小鼠空腹血糖(FBG)。末次给药后取材,测定小鼠血清和肝组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性和丙二醛(MDA)含量。结果:与空白组比较,模型组小鼠的FBG显著升高,而血清和肝组织中SOD和GSH-Px的活性显著降低,MDA含量升高。与模型组比较,鸡矢藤给药组能显著降低小鼠血糖(P<0.01)。经鸡矢藤提取物治疗后,血清和肝组织中的SOD和GSH-Px活性明显升高,MDA含量有所下降(P<0.05,P<0.01)。结论:鸡矢藤提取物能降低糖尿病小鼠血糖,其机制可能与提高机体肝组织的抗氧化能力有关。
Objective: To study the effect of Fructus Chinense (EPS) on the blood glucose and anti-oxidative effects of streptozotocin (STZ) -induced diabetic mice. Methods: Diabetic mice were established by injecting 120 mg · kg-1 STZ into tail vein of mice. Diabetic mice were randomly divided into five groups of 10 animals each: model group, metformin group, low, medium and high dose chicken (2.5,5,10 g · kg-1), the other set 10 Only normal mice served as blank control group. The mice were given gavage for 14 days and the fasting blood glucose (FBG) of mice was measured before and after 7 and 14 days. After the last administration, the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA) in the serum and liver of the mice were determined. Results: Compared with the blank group, FBG in the model group was significantly increased, while the activities of SOD and GSH-Px in serum and liver tissue were significantly decreased, and the content of MDA was increased. Compared with the model group, chicken rotiflora administration group can significantly reduce the blood glucose (P <0.01). The results showed that the activities of SOD and GSH-Px in serum and liver tissue were significantly increased and the content of MDA was decreased (P <0.05, P <0.01). CONCLUSION: Chicken rotundifolia extract can reduce blood glucose in diabetic mice, and its mechanism may be related to improving the antioxidant capacity of liver tissue.