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在pH1．5～2．0酸性溶液中，2．3－二氨基萘选择性地与四份硒离子反应，生成4，5－苯并基硒脑绿色荧光物质，用环已烷萃取，其荧光强度与Se4+的含量成正比，可定量测定硒的含量。
In pH1.5 ~ 2.0 acidic solution, 2.3-diaminonaphthalene selectively reacted with four selenium ions to generate 4,5-benzo-seleno-brain green fluorescent substance, extracted with cyclohexane, Fluorescence intensity is proportional to the content of Se4 +, quantitative determination of selenium content.