利用发根农杆菌Agrobacterium rhizogenes A4诱导樱桃番茄Micro-Tom产生毛状根,并在此基础上成功建立了丛枝菌根(AM)真菌根内球囊霉Glomus intraradices与转移Ri T-DNA番茄离体毛状根的双重培养体系。该真菌侵染14d左右,菌丝开始形成多级分支,17-21d时,一些菌丝顶端膨大,长出新生孢子。接种后3个月时,每皿孢子数量达到600-800个。新形成的孢子无需休眠,可直接侵染番茄离体根。成功的番茄双重培养为番茄菌根生理分子机制的研究提供了理想的实验体系。 The hairy roots were induced by Agrobacterium rhizogenes A4, and the AM fungi Glomus intraradices and RiT-DNA tomato were successfully established. Body hairy root dual culture system. The fungus infected about 14d, hyphae began to form a multi-level branch, 17-21d, some mycelial tip dilated, grow conidia. Three months after inoculation, the number of spores reached 600-800. The newly formed spores do not need dormancy and can directly infect the isolated roots of tomato. Successful double tomato cultivation provides an ideal experimental system for the study of the physiological molecular mechanism of mycorrhiza.