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随着杂交瘤技术的发展,各类单克隆抗体(McAb)相继问世,为鉴别其Ig类别及进一步提纯均需用IgM抗血清。制备该血清用IgM抗原提纯较为困难,需经多种纯化步骤
With the development of hybridoma technology, a variety of monoclonal antibodies (McAb) have come out in succession, in order to identify their Ig categories and further purification are required IgM antiserum. The preparation of this serum is more difficult to purify with IgM antigen and requires several purification steps