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目的探讨表达主要组织相容性复合体(MHC)Ⅱ类分子的甲状腺细胞如何发挥抗原递呈功能。方法分离表达MHCⅡ类分子的转基因鼠和对照小鼠的甲状腺细胞,免疫磁珠方法分离其自身T细胞,在体外共同培养,并加入抗CD28抗体。免疫荧光染色,流式细胞分析检测T细胞的增殖和活化。ELISA方法检测培养上清的细胞因子浓度。结果单纯表达MHCⅡ类分子的转基因小鼠甲状腺细胞不能刺激自身T细胞的增殖和活化,然而当加入抗CD28抗体后,表达MHCⅡ类分子的甲状腺细胞能刺激自身T细胞的增殖、活化及细胞因子γ干扰素(IFN-γ)的分泌,而T细胞对野生型小鼠的甲状腺细胞无反应。结论加入协同刺激信号后,表达MHCⅡ类分子的甲状腺细胞也可刺激T细胞增殖活化及分泌细胞因子,具有递呈抗原的能力。
Objective To investigate how thyrocytes that express major histocompatibility complex (MHC) class II molecules exert antigen presenting function. Methods Thyroid cells from transgenic mice and control mice expressing MHC class II molecules were isolated and their T cells were separated by immunomagnetic beads and cultured in vitro with anti-CD28 antibody. Immunofluorescence staining and flow cytometry were used to detect T cell proliferation and activation. ELISA method to detect the concentration of cytokines in culture supernatant. Results Thyroid cells expressing MHC class II could not stimulate the proliferation and activation of T cells. However, when anti-CD28 antibody was added, thyroid cells expressing MHC class II could stimulate the proliferation and activation of T cells and cytokine γ Interferon (IFN-γ) secretion, while T cells on wild-type mice did not respond to thyroid cells. Conclusions Thyroid cells expressing MHC class Ⅱ molecules can stimulate the proliferation and activation of T cells and secrete cytokines with the ability to present antigens after adding costimulatory signals.