目的:观察高糖条件下VEGF mRNA,EPO mRNA和EPORmRNA在体外培养的Mller细胞中的表达情况。方法:胰蛋白酶将新生小鼠视网膜组织吹打消化后,制成单细胞悬液,体外培养Mller细胞,RT-PCR测定高糖条件下视网膜Mller细胞VEGF,EPO和EPOR基因的表达。结果:成功获得视网膜Mller细胞,传代后90%以上的细胞呈兔抗鼠谷氨酰胺合酶(GS)染色阳性。Mller细胞VEGF mRNA,EPO mRNA和EPOR mRNA在高糖条件下表达升高,且呈浓度依赖性(P<0.05),但糖浓度50mmol/L组较40mmol/L组差异无统计学意义,无明显时间依赖性。结论:Mller细胞在高糖条件下VEGF,EPO,EPOR的表达增加。 Objective: To observe the expression of VEGF mRNA, EPO mRNA and EPOR mRNA in cultured Müller cells under high glucose conditions. Methods: The mouse retinal tissue was trypsinized by beating and digesting to form a single cell suspension. Mller cells were cultured in vitro. The expression of VEGF, EPO and EPOR genes in retinal Mller cells were detected by RT-PCR. Results: The retinal Mller cells were successfully obtained, and more than 90% of the cells were positive for rabbit anti-mouse glutamine synthase (GS) staining after passage. The expression of VEGF mRNA, EPO mRNA and EPOR mRNA in Mller cells were increased in a concentration-dependent manner in a concentration-dependent manner (P <0.05), but there was no significant difference between the 50 mmol / L and 40 mmol / L glucose groups No significant time dependence. Conclusion: The expression of VEGF, EPO and EPOR increased in Mller cells under high glucose condition.