寻找各种过敏原是诊断过敏性疾病的关键,至今为止还没有一种非常完善的方法。本文用阿利新兰染色的嗜硷细胞脱颗粒体外试验(HBDT)具有准确、快速、简便、安全等优点。在临床上有一定意义。材料病人肝素抗凝血和血库献血员肝素抗凝血、稀释的药物和其它含蛋白类抗原。阿利新兰染液和特殊密度的嗜硷细胞分离液。方法为肝素抗凝血3ml与等量pH7.6Hepes缓冲液棍和,平铺于2ml聚蔗糖-泛影葡胺分离液(比重:1.085)2.500rpm离心20分钟。吸取中间细胞层加入5 Looking for a variety of allergens is the diagnosis of allergic diseases, the key, so far there is no perfect method. In this paper, Alizarin blue staining of alkaloidal cell degranulation in vitro (HBDT) with accurate, fast, simple, safe and so on. In clinical significance. Materials Patients Heparin anticoagulants and blood bank donors Heparin anticoagulants, diluted drugs and other protein-containing antigens. Alli New Zealand dye and special density of alkaline cell separation fluid. Methods: Heparin anticoagulant 3ml with the same amount of pH7.6Hepes buffer stick and tiled in 2ml Ficoll-diatrizoate meglumine (specific gravity: 1.085) 2.500rpm for 20 minutes. Draw in the middle cell layer add 5