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氨基酸离子液体(AAILs)由于其独特的化学和物理特性,特别是它突出的生物相容性和优异的绿色特性,已经引起了广泛的关注.本文设计了一种新型的基于氨基酸离子液体和碳纳米管(CNTs)复合物的电化学传感界面.其中,氨基酸离子液体被用作一种新型的葡萄糖氧化酶(GOD)溶剂.通过将碳纳米管修饰的玻碳(GC)电极浸泡在含有葡萄糖氧化酶的氨基酸离子液体溶液中,就可以方便的获得GOD-AAILs/CNTs/GC电极.我们研究了葡萄糖氧化酶在GOD-AAILs/CNTs/GC电极上的直接电化学,获得了一对可逆的氧化还原峰.同时固定在电极上的葡萄糖氧化酶仍然保持了它们的生物活性以及催化溶解氧还原的能力.在氨基酸离子液体和碳纳米管的协同作用下,该GOD-AAILs/CNTs/GC电极展现了对葡萄糖良好的电催化活性,其检测线性范围为0.05～0.8mmol/L,检测限为5.5μmol/L(S/N=3).尤其该传感器显示了良好的稳定性以及排除常见共存物尿酸和抗坏血酸干扰的能力.因此,对于氧化还原酶的直接电化学以及第三代酶传感器的制作来说,AAILs/CNTs复合物将会成为一种很好的生物相容性材料.
Amino acid ionic liquids (AAILs) have attracted a great deal of attention because of their unique chemical and physical properties, especially their outstanding biocompatibility and excellent green properties.In this paper, a novel amino acid ionic liquid (AAILs) Nanotubes (CNTs) composite electrochemical sensing interface in which the amino acid ionic liquid is used as a new type of glucose oxidase (GOD) solvent by carbon nanotubes modified glassy carbon (GC) electrode immersed in containing GOD-AAILs / CNTs / GC electrode can be easily obtained in the solution of amino acid ionic liquid of glucose oxidase.We studied the direct electrochemical of glucose oxidase on GOD-AAILs / CNTs / GC electrode and obtained a pair of reversible Of the redox peaks.At the same time, glucose oxidase immobilized on the electrode still maintained their biological activity and the ability of catalyzing dissolved oxygen reduction.In the synergistic effect of amino acid ionic liquids and carbon nanotubes, the GOD-AAILs / CNTs / GC The electrode exhibited good electrocatalytic activity for glucose with a linear range of 0.05-0.8 mmol / L and a detection limit of 5.5 μmol / L (S / N = 3) .In particular, the sensor showed good stability As well as the ability to rule out the common coexisting uric acid and ascorbic acid, AAILs / CNTs complexes will therefore be a good biocompatible for the direct electrochemical redox enzyme and the third generation of enzyme sensors material.