应用混合采样法构建了光敏核不育水稻育性转换敏感期可育和不育幼穗代表群,并对其进行了cDNA-RAPD分析.结果表明:混合采样法是可行的,可以避免常规采样引起的误差.在筛选的150个随机引物中,有83个随机引物扩增的条带完全相同,有34个随机引物扩增的带型相同,但存在扩增量的差异,有33个随机引物可在可育和不育幼穗cDNA间扩增出有差异的cDNA条带,多态性比率为22%.研究还发现,除了不育基因差异表达外,水稻中可能还存在许多不育相关基因,使差异cDNA片段复杂化. A mixed population sampling method was used to construct the representative population of fertile and sterile young fertile plants in sensitive genic male-sterile rice (Oryza sativa L.). The results of the cDNA-RAPD analysis showed that the mixed sampling method was feasible and could avoid the conventional sampling Of the random primers.Among the 150 random primers screened, 83 random primers amplified the same bands, and 34 random primers amplified the same bands, but there was a difference in the amplification amount. There were 33 randomized Primers can amplify differentially expressed cDNA bands between fertile and sterile young spike cDNAs with a 22% polymorphism. The study also found that in addition to the differential expression of sterile genes, there may be many infertility in rice Related genes complicate differences in cDNA fragments.