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目的 观察缺血预处理 (IPC)对肝脏缺血再灌注 (I/R)损伤后肝功能的保护作用。方法 大鼠48只随机分为I/R组和IPC组。观测缺血后的肝脏谷丙转氨酶 (ALT)、乳酸脱氢酶 (LDH )、髓过氧化物酶(MPO)及肝组织的病理变化。结果 ALT在I/R组各时相点比缺血前升高 47%～ 5 8% ,而IPC组仅在缺血后2 4h升高 2 0 % ;LDH在I/R组与缺血前比较 1、6、2 4h均升高 (P <0 .0 1) ,IPC组仅在缺血 6h较缺血前升高 (P<0 .0 5 ) ;I/R组MPO与IPC组比较有显著增高 (P <0 .0 5 )。病理检查IPC组比I/R组肝细胞损伤减轻、坏死范围小。结论 IPC可以减轻再灌注对肝脏的损伤 ,减少LDH的漏出和MPO的生成 ,而保护肝功能
Objective To observe the protective effect of ischemic preconditioning (IPC) on liver function after hepatic ischemia-reperfusion (I / R) injury. Methods Forty-eight rats were randomly divided into I / R group and IPC group. The pathological changes of hepatic alanine aminotransferase (ALT), lactate dehydrogenase (LDH), myeloperoxidase (MPO) and liver tissue were observed after ischemia. Results Compared with pre-ischemic group, ALT increased 47% ~ 58% in I / R group and 20% in IPC group 24 h after ischemia. (P <0.01), the IPC group only increased 6h after ischemia compared with that before ischemia (P <0.05); MPO in I / R group was significantly higher than that in IPC group There was a significant increase (P <0.05). Pathological examination of IPC group than the I / R group of liver cell lesion reduced, a small area of necrosis. Conclusion IPC can reduce the damage of liver caused by reperfusion, reduce the leakage of LDH and the production of MPO, and protect liver function