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采用毒力测定的方法测定了长沙地区斜纹夜蛾种群对甲维盐、氯氟氰菊酯的抗性水平。同时,测定了其体内4种代谢解毒酶(羧酸酯酶、乙酰胆碱酯酶、谷胱甘肽转移酶、多功能氧化酶)和淀粉酶的活性。结果表明,湖南长沙地区的斜纹夜蛾田间种群对甲维盐和氯氟氰菊酯这两种杀虫剂,相对于敏感种群产生的抗性倍数分别为30.35倍与47.28倍。与敏感种群相比,长沙地区斜纹夜蛾种群的代谢解毒酶均有显著的提高,表明4种代谢酶在斜纹夜蛾对甲维盐与氯氟氰菊酯的抗性中起到了重要的作用。长沙田间斜纹夜蛾种群的淀粉酶活力显著高于敏感种群,说明杀虫剂可能在一定程度上激活了淀粉酶的活力。
The method of virulence determination was used to determine the level of resistance to methionine salt and cyhalothrin in the Spodoptera litura population in Changsha area. Meanwhile, the activities of four kinds of metabolic detoxification enzymes (carboxylesterase, acetylcholinesterase, glutathione transferase, multifunctional oxidase) and amylase were measured. The results showed that the two insecticides against the carbaryl and cyhalothrin in the field population of S. litura in Changsha, Hunan province were 30.35 times and 47.28 times more sensitive to the susceptible population respectively. Compared with the susceptible population, the metabolic detoxification enzymes of S. litura population were significantly increased in Changsha, indicating that the four metabolic enzymes played an important role in the resistance of S. litura to the carbaryl and cyhalothrin . The amylolytic activity of the Spodoptera exigua population in Changsha was significantly higher than that of the susceptible population, indicating that insecticides may activate the activity of amylase to a certain extent.