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大豆疫霉根腐病是危害大豆生产的世界范围的毁灭性病害,毒素在其致病过程中起着重要的作用。本文对大豆疫霉根腐病菌毒素胁迫下抗感不同大豆品种根、茎、叶中多酚氧化酶(PPO)活性的变化进行了初步研究,结果表明:适宜浓度的毒素(稀释100倍,浓度为0.0897mg/mL)处理后,抗病品种根、茎和叶中PPO活性在病程的大部分阶段与对照相比都升高,并且根中的PPO比茎叶中的PPO反应更敏感;而感病品种在整个病程中虽然在某些阶段较对照有一定的提高,但幅度不大,在病程其他阶段PPO下降幅度远大于升高幅度。而浓度相对较高的毒素(稀释50倍,浓度为0.1794mg/mL)处理后抗感品种根、茎和叶中PPO活性的变化较稀释100倍浓度毒素幅度小。
Phytophthora sojae is a worldwide devastating disease that endangers soybean production, and toxins play an important role in its pathogenicity. In this paper, the changes of polyphenol oxidase (PPO) activity in roots, stems and leaves of soybean cultivars infected with Phytophthora infestans under different toxin stress were studied. The results showed that the optimum concentration of toxins (diluted 100 times, 0.0897mg / mL), PPO activities in roots, stems and leaves of disease-resistant cultivars increased at most stages of the course of disease and the PPO in roots was more sensitive than PPO in stems and leaves. However, Although the susceptible varieties in certain stages of the disease in some stages compared with the control of a certain increase, but the magnitude is not large, in the other stages of the PPO decline is much larger than the increase rate. However, the changes of PPO activity in roots, stems and leaves of resistant varieties were lower than that of 100 times diluted concentrations of toxins (50-fold diluted, 0.1794 mg / mL).