肝脏灌流技术约有100多年的历史。早在19世纪,就有一些科学家用肝脏灌流技术进行三大物质代谢的研究。离体肝脏灌流技术(isolated liver perfusion)由英国生理学家Trowell1942年创立,1951 Miller等人改进了灌流方法,首次以此技术成功地进行了血浆蛋白质生物合成的研究。但是,离体肝脏灌流技术直到六十年代才较广泛地被采用。近年来,离体肝脏灌流技术得到了迅速发展,已普遍地应用 Liver perfusion technique for about 100 years of history. As early as the 19th century, some scientists used liver perfusion techniques to study the three major metabolites. Isolated liver perfusion was initiated in 1942 by the British physiologist Trowell. In 1951, Miller et al. Improved the perfusion method. For the first time, this study successfully performed the plasma protein biosynthesis. However, ex vivo liver perfusion technology was not widely adopted until the 1960s. In recent years, the ex vivo liver perfusion technique has been rapidly developed, has been widely used