针对严重危胁烤烟大田生产的赤星病病害，培育抗病品种。本研究以烤烟品种Ｎｃ８９为受体，Ｃｖ８７为供体，采用浸苗法将供体总ＤＮＡ导入受体，通过表型、抗病性和过氧化物酶同工酶检测，筛选抗病变异材料。研究结果表明，外源总ＤＮＡ直接导入可有效转移株高、株型、叶色、叶面积大小、生育期及抗病性等性状，并获得了较高转化率（７．１０％）。 Targeted at the serious threat to the production of flue-cured tobacco leaf spot disease, cultivate disease-resistant varieties. In this study, tobacco Nc89 as acceptor and Cv87 as donor were used to introduce total donor DNA into the recipient. The phenotype, disease resistance and peroxidase isozymes were used to screen the disease-resistant materials . The results showed that direct introduction of total exogenous DNA could effectively transfer plant height, plant type, leaf color, leaf area size, growth period and disease resistance, and obtained a higher conversion rate (7.10%).