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目的分析天然成分松油烯-4-醇对人宫颈癌HeLa细胞增殖活性的影响。方法体外培养人宫颈癌细胞HeLa,经不同浓度(0.001%～0.010%)松油烯-4-醇作用24h后,倒置显微镜观察细胞形态变化,四甲基偶氮唑蓝(MTT)比色法检测细胞活性。结果细胞形态明显改变,可见显著凋亡的特征;在药物浓度为0.001%～0.006%时,随着浓度增加,抑制率从14.0%上升至95.1%,松油烯-4-醇对HeLa细胞的抑制作用明显增强;在药物浓度为0.006%～0.01%时,抑制率维持在95.1%～96.3%,抑制作用未见增强。结论天然成分松油烯-4-醇对人宫颈癌HeLa细胞增殖有明显的抑制作用。
Objective To analyze the effect of natural ingredient terpinen-4-ol on the proliferation of human cervical cancer HeLa cells. Methods Human cervical cancer HeLa cells were cultured in vitro. The morphology of the cells was observed by inverted microscope after treated with different concentrations of terpinen-4-ol (0.001% -0.010%) for 24 h. MTT assay Cell activity was measured. Results The morphological changes of cells were observed. The apoptotic characteristics of HeLa cells were observed when the drug concentration was 0.001% ~ 0.006%, with the increase of concentration, the inhibition rate increased from 14.0% to 95.1% The inhibitory effect was obviously enhanced. When the drug concentration was 0.006% ~ 0.01%, the inhibition rate was maintained at 95.1% ~ 96.3%, and the inhibition was not enhanced. Conclusion Terpinen-4-ol, which is a natural component, obviously inhibits the proliferation of human cervical cancer HeLa cells.